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3x ere tata luc  (Addgene inc)


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  • 94

    Structured Review

    Addgene inc 3x ere tata luc
    3x Ere Tata Luc, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 88 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/3x+ere+tata+luc/3X+ERE+TATA+luc+(Plasmid+%2311354)/pmc12992960-49-0-9
    Average 94 stars, based on 88 article reviews
    3x ere tata luc - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Estrogenic activity of E2-conjugated GLP-1 is mediated by intracellular endolysosomal acidification and estrone metabolism
    Article Snippet: ER-mNeonGreen was a gift from Dorus Gadella (Addgene plasmid # 137804). mEmerald-LaminB1-10, mEmerald-Rab4a, mEmerald-Rab7a and mEmerald-Rab11a were gifts from Michael Davidsons (Addgene plasmid # 54140, # 54242, # 54244, # 54245). pCMV-hERα was a gift from Elizabeth Wilson (Addgene plasmid # 101141) as originally published by Professor David J Shapiro [ ]. pcDNA Flag ERβ was a gift from Harish Srinivas (Addgene plasmid # 35562). .. 3X ERE TATA luc was a gift from Donald McDonnell (Addgene plasmid # 11354). pcDNA3.1(+)-ExRai-AKAR2 was a gift from Jin Zhang (Addgene plasmid # 161753). pCMV-GFP was a gift from Connie Cepko (Addgene plasmid # 11153). .. Plasmids eGFP-Rab35, eGFP-Rab22, eGFP-Rab10, eGFP-Rab1a, eGFP-Rab3a were gifts from Marci Scidmore (Addgene plasmid # 49552, # 49600, # 49472, # 49467, # 49542).

    Article Title: Estrogen Receptor Βeta Stimulation as a Possible Novel Therapeutic Target for Cutaneous T-Cell Lymphoma.
    Article Snippet: The next day, cells were transfected with 1.5 μg ERE firefly luciferase construct and 0.5 μg TK Renilla vector per well (Promega, Madison, WI, USA) using the Lipofectamine 2000 transfection reagent (Invitrogen, Waltham, MA, USA) in OptiMEM without phenolred (Thermo Fisher Scientific, Waltham, MA, USA). .. 3X ERE TATA luc was a gift from Donald McDonnell (Addgene plasmid # 11354; http://n2t.net/addgene:11354; RRID:Addgene 11354). ..

    Article Title: Optogenetic manipulation of estrogen receptor signaling to improve estrogen deficiency
    Article Snippet: .. 3X ERE TATA luc , Hall et al. , Addgene plasmid # 11354. ..

    Article Title: Estrogen receptors α/β exhibit distinct intermolecular interactions to mediate gene regulatory events.
    Article Snippet: Aim: Estrogen receptors (ERα and ERβ) are intracellular transcription factors that regulate gene expression when bound by estrogens.. Although their roles in cellular functions and the onset of disease are relatively well-documented, their interactions with mitotic chromatin in the context of ‘mitotic bookmarking’ remain largely unexplored.. The current study revealed distinct mechanisms underlying mitotic chromatin retention of ERα and ERβ, emphasizing structural and functional determinants of ER-chromatin association.

    Activity Assay:

    Article Title: Small molecule conjugates with selective estrogen receptor β agonism promote anti-aging benefits in metabolism and skin recovery
    Article Snippet: .. Plasmids used for reporter activity assay were 3x-ERE-TATA-Luc (Addgene #11354), pCMV-hERa (Addgene #101141), and pcDNA-Flag-hERb (Addgene #35562). .. Luciferase activity was assessed using a Dual-Luciferase Reporter Assay System (Promega, E1980) and measured with a plate reader (Tecan Infinite F200).

    Article Title: Small molecule conjugates with selective estrogen receptor β agonism promote anti-aging benefits in metabolism and skin recovery
    Article Snippet: .. Plasmids used for reporter activity assay were 3x-ERE-TATA-Luc (Addgene #11354), pCMV-hERa (Addgene #101141), and pcDNA-FlaghERb (Addgene #35562). .. Luciferase activity was assessed using a Dual-Luciferase Reporter Assay System (Promega, E1980) and Please cite this article as: Zahr Tarik et al., Small molecule conjugates wit metabolism and skin recovery, Acta Pharmaceutica Sinica B, https://doi.org/1 measured with a plate reader (Tecan Infinite F200).

    Infection:

    Article Title: Inducible estrogen receptor alpha in normal breast epithelial cells demonstrate estrogen receptor-dependent DNA damage
    Article Snippet: .. HBEC- ESR1 cells were infected with 3X ERE-TATA-Luc (Addgene, #11354) and Renilla using Lipofectamine 2000. ..

    Transfection:

    Article Title: Estrogen receptors α/β exhibit distinct intermolecular interactions to mediate gene regulatory events.
    Article Snippet: Aim: Estrogen receptors (ERα and ERβ) are intracellular transcription factors that regulate gene expression when bound by estrogens.. Although their roles in cellular functions and the onset of disease are relatively well-documented, their interactions with mitotic chromatin in the context of ‘mitotic bookmarking’ remain largely unexplored.. The current study revealed distinct mechanisms underlying mitotic chromatin retention of ERα and ERβ, emphasizing structural and functional determinants of ER-chromatin association.



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